Today, I finally achieved independence! Jian Xiang now leaves me alone to my own work, and I can mill about the lab without his suspicion. I am currently running RT-PCR with GAPDH (a housekeeping gene) and altering the amounts of the HCC DNA so that expression levels for GAPDH would be constant against all HCC cell lines. This is to normalize gene expression data so that it is reliable and accurate. I can't wait to finish this initial step and move onto the bulk of the project! Today was also a day of compliments. The PI visited our lab and commented to the others, "Christine's fairly independent huh?" while the postdoctoral fellows complimented me in Mandarin. I could not catch much of it because I can not understand Mandarin, but I believe they were discussing my intelligence and work ethic. Of course, my ego felt warm and fuzzy after having such a good day in the lab, but it was destroyed when I made the mistake of leaving my materials out on the bench. It was my first mistake yet, and by the time I realized it, I was already on the MRT at Harbour Front station. I quickly rushed back to NCCS to place the DNA, Master Mix, and primers back into the -20 degree Fahrenheit fridge. At this point, I had wasted a sufficient amount of time and by the time I arrived back to PGP, it was 7:30 PM. I missed dinner with the others once again and we planned to meet downstairs in the lounge at 9 PM, so I only had a short time window to eat. Thankfully, I brought food home from the NCCS Meeting and I was able to microwave it for dinner. The food was not the best, but at least I satisfied my need for food!
Once we were downstairs, we booked our hostel for Thailand. I can't believe the trip is already coming up!
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